Method of staining and identifying cells and compositions thereo

Details Method of staining and identifying cells and compositions thereo Method of staining and identifying cells and compositions thereo

1985-08-13

1987-12-22

Warden, Robert J.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

424 71, 435 29, 435 34, 435 39, 534611, G01N 100, G01N 3348

Patent

active

047146060

ABSTRACT:
This invention is directed to the cytology of blood, bone marrow and lymph node cells and the method of differentiating, identifying and enumerating said cells among a plurality of cells of hematopoietic origin. More specifically, the invention is directed to the use of a single cationic sulfur-containing azo dye in solution capable of staining said cells in a fixative. The dye is a water soluble quaternary azo dye identified in the Color Index as Basic Blue 41 capable of staining a plurality of cells including malignant cells of hematopoietic origin wherein the stained cells acquire individual color characteristics permitting the differentiation, identification and enumeration of each stained cell. The stained cells obtained by the process of this invention have individual color characteristics which permit the identification and differentiation by use of various instruments including, for example, an image analyzer, microscope, photomicroscope, and the like.

REFERENCES:
patent: 3578386 (1971-05-01), Kalopissis et al.
patent: 4400370 (1983-08-01), Kass
patent: 4500509 (1985-02-01), Kass
patent: 4581223 (1986-04-01), Kass
Color Index, Revised Third Edition, vol. 6, (1975), pp. 5088 and 6392.
Williams, et al., Methods in Immunology & Immunochemistry, vol. 2, Academic Press, New York, 1968, pp. 365-380.

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