Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Plant proteins – e.g. – derived from legumes – algae or...
Patent
1991-03-15
1992-04-07
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Plant proteins, e.g., derived from legumes, algae or...
424 89, 530324, 530412, 530414, 530417, 530820, 530825, 530826, 530824, A61K 3929, C07K 318, C07K 328
Patent
active
051029898
ABSTRACT:
Recombinant hepatitis B virus surface proteins produced in recombinant host cells are rapidly and efficiently purified from either cell extracts in a high pH buffer, or from heated whole cells at neutral pH. The host cell extracts or whole cells are heat treated, cooled and in the case of high pH extract, the pH is reduced. The surface proteins are then absorbed onto wide pore silica followed by elution and concentration. This method eliminates the requisite introduction of protease inhibitors, stabilizes the surface protein and improves product yield.
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Kubek Dennis J.
Sitrin Robert D.
Merck & Co. , Inc.
Pfeiffer Hesna J.
Schain Howard E.
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