Method of sorting cells

Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...

Reexamination Certificate

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C600S033000

Reexamination Certificate

active

10221241

ABSTRACT:
A method of sorting cells, particularly, but not exclusively, sperm cells, is described. The method comprises the use of a surface decontamination medium to strip the cells of any extra-cellular surface contamination and to then subject to stripped cells to a charge based separation.

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patent: 4225405 (1980-09-01), Lawson
Ijaz et al. Journal of Dairy Science. 1989, vol. 72, No. 10, pp. 2683-2690.
Balerna et al. “Human Sperm Processing”. Prog. Reprod. Biol. Med., 1994, vol. 16, pp. 45-75.
Manger et al. “Effect of sperm motility on separation of bovine x- and Y- bearing spermatozoa by means of free-flow electrophoresis”ANDROLOGIA, vol. 29, No. 1, Jan. 1997-Feb. 1997pp. 9-15.
Ishijima et al. “Zeta potential of human x- and y-bearing sperm”International Journal of Andrology, vol. 14, No. 5, Oct. 1991, pp. 340-347.
Engelmann et al. “Separation of human x and y spermatozoa by free flow electrophoresis”Gamete Research, vol. 19, No. 2, 1998, pp. 151-160.
Blottner et al. “Enrichment of bovine x and y spermatozoa by free-flow electrophoresis”Journal of Veterinary Medicine Series A, vol. 41, No. 6. Aug. 1994, pp. 466-474.

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