Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.
Patent
1986-08-25
1987-09-15
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Hormones, e.g., prolactin, thymosin, growth factors, etc.
424108, 514 2, 514 21, 530397, 530410, 530808, 530825, C07K 1500, C07K 308
Patent
active
046940731
ABSTRACT:
A method for solubilization and naturation of somatotropin protein from refractile bodies of host cells is disclosed. The method embraces the discovery that an aqueous solution of urea, or dimethylsulfone, or mixtures of urea and dimethylsulfone, can be effectively used to solubilize refractile bodies containing such somatotropin protein. Once solubilized, somatotropin protein can be natured in an aqueous solution of urea, or dimethylsulfone, or mixtures of urea and dimethylsulfone, by contacting the solution with a mild oxidizing agent for a time sufficient to result in the formation of the disulfide bonds. Naturation can efficiently occur even at high protein concentration, in an impure preparation and in the absence of reducing agent.
REFERENCES:
patent: 4342832 (1982-08-01), Goeddel et al.
patent: 4512922 (1985-04-01), Jones et al.
Archives of Biochemistry & Biophysics, 138 (1970), 338-346, Bewley et al.
Methods in Enzymology, XXXIV, 67, 544-547 (1974), Sluyterman et al.
FEBS Letters, 80, No. 2, 408-412 (1977), Kohl et al.
Biochemistry, 2nd ed. (1981), Stryer, pp. 32-35.
Bentle Larry A.
Mitchell James W.
Shimamoto Grant T.
Storrs Stephen B.
Beck George R.
Beusen Jon H.
Monsanto Company
Schain Howard E.
Stanley Howard C.
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