Method of somatotropin naturation

Details Method of somatotropin naturation Method of somatotropin naturation

1985-02-22

1987-03-24

Phillips, Delbert R.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Separation or purification

530420, C07K 102

Patent

active

046526307

ABSTRACT:
Methods of solubilization and naturation of somatotropin protein from refractile bodies of host cells are disclosed. The solubilization methods embrace the discovery that an aqueous urea solution can be effectively used to solubilize refractile bodies containing such somatotropin protein. Surprisingly, it has been further found that once solubilized, such somatotropin protein can be natured in the urea solution by contacting the solution with a mild oxidizing agent for a time sufficient to result in the formation of the disulfide protein. Naturation can efficiently occur even at high protein concentration, in an impure preparation and in the absence of reducing agent.

REFERENCES:
patent: 3845204 (1974-10-01), Grant
Lowenstein, "Methods in Enzymology" vol. 13 (1969), 90-99.
Stryer, Biochemistry (1982), 33-35.

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