Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1995-08-30
1998-12-01
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 71, 435 912, C12Q 170, C12Q 168
Patent
active
058436405
ABSTRACT:
In one aspect, the present invention provides an in situ process of simultaneously detecting a specific predetermined nucleic acid sequence and a specific predetermined cellular antigen in the same cell. In accordance with that process, the antigen is labeled with a biotin- or DNP-tagged antibody that specifically immunoreacts with the antigen, the specific nucleic acid sequences in the cell are amplified, the amplified nucleic acid sequences are labeled with a fluorescently-tagged nucleic acid probe that specifically hybridizes to the amplified nucleic acid sequences, and the labeled nucleic acid sequences and labeled cellular antigen are detected.
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Patterson Bruce
Till Michelle
Wolinsky Steven
Atzel Amy
Jones W. Gary
Northwestern University
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