Method of sequencing [short] oligonucleotides

Details Method of sequencing [short] oligonucleotides Method of sequencing [short] oligonucleotides

1994-01-26

1996-06-11

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435810, 436501, 436 63, 536 221, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, 935 88, C12Q 168

Patent

active

055254705

ABSTRACT:
Disclosed is a method for determining the nucleotide sequence of a target oligonucleotide. In this method a single-stranded ligation product is prepared which contains a target oligonucleotide-to-be sequenced and an auxiliary oligonucleotide. A primer complementary to a portion of the auxiliary oligonucleotide and having a label covalently attached thereto is annealed to the auxiliary oligonucleotide portion of the ligation product. The primer is extended with chain-extending nucleoside triphosphates and chain-terminating nucleoside triphosphates in the presence of a polymerase to yield a plurality of primer extension products. These extension products are then separated on the basis of their base length; and the nucleoside sequence of the target oligonucleotide is determined from the mobilities of the primer extension products obtained during their separation.

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