Method of sequencing peptides

Details Method of sequencing peptides Method of sequencing peptides

1986-04-28

1987-05-12

Richman, Barry S.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436161, 436178, G01N 3000, G01N 3368

Patent

active

046650377

ABSTRACT:
A method of sequencing polypeptides is disclosed utilizing liquid-solid affinity chromotography. The method utilizes a reagent reactive at one position with the amino moiety of a terminal amino acid, and reactive at a second position with boronic acid. The reagent is coupled to the terminal amino acid. A scavenger molecule having a sulfhydryl group and an amine group reacts with the excess reagent and the excess scavenger and scavenger-reagent complex are removed on an immobilized organomercurial column. The terminal amino acid is cleaved from the polypeptide, and coupled to an immobilized boronic acid column. The amino acid is removed from the column and identified and the remainder polypeptide is recycled.

REFERENCES:
patent: 4065412 (1977-12-01), Dreyer
patent: 4548904 (1985-10-01), Kent et al.
patent: 4603114 (1986-07-01), Hood et al.
Inglis et al, J. of Biochem. and Biophys. Methods, vol. 4, pp. 279-285, 1981.
"Methods of Biochemical Analysis", vol. 26, edited by Dr. David Glick, pp. 234-236, published by John Wiley & Sons, Inc., 1980.

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