Method of sequencing DNA

Chemistry: analytical and immunological testing – Process or composition for determination of physical state... – Corrosion resistance or power

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C536S022100

Reexamination Certificate

active

07459311

ABSTRACT:
The present invention provides a method of identifying a base at a target position in a sample nucleic acid sequence, said method comprising: subjecting a primer hybridised to said sample nucleic acid immediately adjacent to the target position, to a polymerase primer extension reaction in the presence of a nucleotide, whereby the nucleotide will only become incorporated if it is complementary to the base in the target position, and determining whether or not said nucleotide is incorporated by detecting whether Ppi is released, the identity of the target base being determined from the identity of any nucleotide incorporated, wherein, where said nucleotide comprises an adenine base, an α-thio triphosphate analogue of said nucleotide is used, and the Rp isomer of said analogue and/or the degradation products of said analogue are eliminated from the polymerase reaction step.

REFERENCES:
patent: 4654267 (1987-03-01), Ugelstad et al.
patent: 6210891 (2001-04-01), Nyren et al.
patent: 6258568 (2001-07-01), Nyren
patent: 6828100 (2004-12-01), Ronaghi
patent: 2000-55917 (2000-02-01), None
patent: WO 89/09283 (1989-10-01), None
patent: WO 93/23564 (1993-11-01), None
patent: WO 94/28169 (1994-12-01), None
patent: WO 98/13523 (1998-04-01), None
patent: WO 98/28440 (1998-07-01), None
patent: 00-37646 (2000-06-01), None
patent: WO 00/43540 (2000-07-01), None
Yee et al. Biochemistry vol. 18:4116-4120. 1979.
Ronaghi. Analytical Biochemistry vol. 286:282-288. 2000.
Yee et al. Biochemistry vol. 19:4116-4119. 1997.
Suzuki et al. Gene vol. 200:149-156. 1997.
Armstrong et al. (1979) Biochemistry 18:4120-4123.
Romaniuk et al. (1982) Journal of Biological Chemistry 257:7684-7688.
Yee et al. (1979) Biochemistry 18:4116-4120.
Eckstein, Ann. Rev. Biochem., 54: 367-402 (1985).
Le Bel et al., J. Biol. Chem., 255: 1227-1233 (1980).
Reeves et al., Anal. Biochem., 28: 282-287 (1969).
Guillory et al., Anal. Biochem., 39: 170-180 (1971).
Johnson et al., Anal. Biochem., 26: 137-145 (1968).
Cook et al., Anal. Biochem., 91: 557-565 (1978).
Drake et al., Anal. Biochem., 94: 117-120 (1979).
Nyren et al., Anal. Biochem., 151: 504-509 (1985).
Kajiyama et al., Biosci. Biotechnol. Biochem., 58: 1170-1171 (1994).
Onda et al., Biosci. Biotech. Biochem., 60: 1740-1742 (1996).
Ronaghi et al., Science, 281: 363-365 (1998).
Karamohamed et al., Protein Expression and Purification, 15: 381-388 (1999).
Ford et al., Methods in Mol. Biol., 102: 3-20 (1998).
Ronaghi et al., Anal. Biochem., 242: 84-89 (1996).
Chen et al., Proc. Natl. Acad. Sci. USA, 94: 10756-10761 (1997).
Doremus et al., Plant Physiol., 87: 36-40 (1988).
Mizrahi et al., Biochemistry, 24: 4010-4018 (1985).
Outlaw, William H. et.al., “Romoval of Contaminating Nucleoside Diphosphates from Commercial Preparations of Uridine Diphosphoglucose”, Analytical Biochemistry 171, 1988, pp. 104-107, Academic Press, Inc.
Burgers, Peter M. J. et. al., “A Study of the Mechanisms of DNA Polymerase I fromEscherichia coliwith Diastereomeric Phosphorothioate Analogs of Deoxyadenosine Triphosphate”, The Journal of Biological Chemistry, vol. 254, No. 15, Aug. 10, 1979, pp. 6889-6893.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method of sequencing DNA does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of sequencing DNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of sequencing DNA will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4023215

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.