Method of sequencing DNA

Details Method of sequencing DNA Method of sequencing DNA

1993-09-22

1995-04-11

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 935 77, 935 78, C12Q 168, C12P 1934

Patent

active

054057469

ABSTRACT:
The invention relates to a method of sequencing target DNA in which said DNA is provided in double stranded form immobilized on a solid support via one terminus of one of the two strands thereof and is then subjected to strand separation whereby the unattached strand is removed prior to sequencing the immobilized strand. The target DNA, e.g genomic DNA, may initially be amplified by the PCR method using at least one oligonucleotide primer which is either attached to a solid support or carries means for attachment to a solid support.

REFERENCES:
patent: 4734363 (1988-03-01), Dattagupta et al.
patent: 4851331 (1989-07-01), Vary et al.
Hartley et al., Nuc. Acids Res. 10 (13): 4009-4025 (1982).
Rosenthal et al., Nuc. Acids Res. 13 (4): 1173-1184 (1985).
Nucleic Acids Research, vol. 13, No. 15, pp. 5457-5469, 1985 "Separation of complementary strands of plasmid DNA using the biotin-avidin system and its application to heteroduplex . . . " Hajo Delius, et al.
Methods in Enzymology, vol. 155, pp. 301-331, 1987 "Solid-Phase Methods for Sequencing of Oligodeoxyribonucleotides and DNA" Andre Rosenthal, et al.
Nucleic Acids Research, vol. 16 No. 7, pp. 3025-3039, Apr. 11, 1988, "Solid phase DNA sequencing using avidin system" Stefan Stahl, et al.
Nucleosides & Nucleotides, vols. 7(5&6), pp. 629-638, 1988, "Approaches to Solid Phase DNA Sequencing", Thomas Hultman, et al.

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