Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-12-13
1998-06-23
Wolski, Susan
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 72, 435 721, 435 79, 435 792, 435 794, 436501, 436518, 436514, 436526, 436806, 436824, G01N 3353
Patent
active
057703884
ABSTRACT:
Methods are disclosed for separating a component of interest from a mixture containing the component of interest and other components. The method comprises contacting a first liquid medium containing the component of interest and other components with a second liquid medium that is of different density than and/or of different viscosity than the first liquid medium. The contact is carried out in such a way that mixing of the media is minimized or avoided. The component of interest is bound to magnetic particles. The contacted first liquid medium and second liquid medium are subjected to a magnetic field gradient to allow the magnetic particles to migrate into the second liquid medium and separation of the component of interest from other components is realized. Also disclosed are assays employing the present method. Kits for carrying out the present method and assays are also disclosed.
REFERENCES:
patent: 3933997 (1976-01-01), Hersh et al.
patent: 3970518 (1976-07-01), Giaever
patent: 4018886 (1977-04-01), Glaever
patent: 4070246 (1978-01-01), Kennedy et al.
patent: 4115534 (1978-09-01), Ithakissios
patent: 4115535 (1978-09-01), Glaever
patent: 4285819 (1981-08-01), Yen et al.
patent: 4413070 (1983-11-01), Rembaum
patent: 4452773 (1984-06-01), Molday
patent: 4454234 (1984-06-01), Czerlinski
patent: 4523996 (1985-06-01), Charles et al.
patent: 4526681 (1985-07-01), Friedlaender et al.
patent: 4731337 (1988-03-01), Luotola et al.
patent: 4777145 (1988-10-01), Luotola et al.
patent: 4783401 (1988-11-01), Horan et al.
patent: 4868130 (1989-09-01), Hargreaves
patent: 4935147 (1990-06-01), Ullman et al.
patent: 5073341 (1991-12-01), Hargreaves
patent: 5279936 (1994-01-01), Vorpahl
Weaver, et al., "Gel Microdroplets: Rapid Detection and Enumeration of Individual Microorganisms by Their Metabolic Activity", Biotechnology, (1988 ) vol. 6, pp. 1084-1089.
Henning et al., "Isolation of Rat Liver Lysosomes by Loading with Colloidal Gold", Biochim. Biophys. Acta (1974) vol. 354, pp. 114-120.
Dorn et al., "Blood Culture Technique Based on Centrifugation: Developmental Phase", J. Clin. Micro. (1976) vol. 3, pp. 251-257.
Leduc et al., "Visualization of Poly (ADP-ribose) Synthetase Associated with Polynucleosomes by Immunoelectron Microscopy", Biochim. Biophys. Acta (1986) vol. 885, pp. 248-255.
Courtoy, et al., "Shift of Equilibrium Density Induced by 3,3'-Diaminobenzidine Cytochemistry: A New Procedure for the Analysis and Purification of Peroxidase-containing Organelles", J. Cell Biology (1984) vol. 98, pp. 870-876.
Dutton et al., "Iron-Dextran Antibody Conjugates: General Method for Simultaneous Staining of Two Components in High-Resolution Immunoelectron Microscopy", Proc. Natl. Acad. Sci. (1979) vol. 76, pp. 3392-3396.
Ithakissios, et al., "Use of Protein Containing Magnetic Microparticles in Radioassays", Clin. Chem. (1977) vol. 23, pp. 2072-2079.
Molday, et al., "Separation of Cells Labeled with Immunospecific from Dextran Microspheres Using High Gradient Magnetic Chromatography" FEBS (1984) vol. 170, pp. 232-238.
Molday, et al., "Immunospecific Ferromagnetic Iron-Dextran Reagents for the Labeling and Magnetic Separation of Cells", J. Immunol. Meth. (1982) vol. 52, pp. 353-357.
Molday, et al., "Application of Magnetic Microspheres in Labelling and Separation of Cells", Nature (1977) vol. 268, pp. 437-438.
Nargessi, et al., "Solid-Phase Fluoroimmunoassay of Human Albumin in Biological Fluids", Clin. Chim. Acta (1978) vol. 89, pp. 455-460.
Nye, et al., "Solid-Phase, Magnetic Particle Radioimmunoassay", Clin. Chim. Acta (1976) vol. 69, pp. 387-396.
Rosenweig, "Magnetic Fluids", Scien. Amer. (1983) vol. 10, pp. 136-145.
Widder, et al., "Magnetic Protein A Microspheres: A Rapid Method for Cell Separation", Clin. Immunol. and Immunopath. (1979) vol. 14, pp. 395-400.
Baldwin, et al., "Biomat: A Rapid, Self-Washing Immunoassay System with a Microparticulate Solid Phase", Clinical Chemistry (1987) vol. 33:9, pp. 1566-1567.
Freifelder, D., Molecular Biology (1983) Science Book Int'l Publishers, pp. 88-91.
Owen Beckman, Mitch Griffith, Techniques Preparative, Zonal, and Continuous Flow Ultracentrifugation, pp. 3, 10, 11.
Harlow et al., "Antibodies: A Laboratory Manual", Cold Spring Laboratory, (1988), pp. 547-549.
Dade Behring Marburg GmbH
Jordan Leland K
Leitereg Theodore J.
Rosenstock Jerome
Wolski Susan
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