Method of separation employing magnetic particles and second med

Details Method of separation employing magnetic particles and second med Method of separation employing magnetic particles and second med

1989-12-22

1994-01-18

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 792, 435 71, 435 793, 435 794, 435 795, 436501, 436512, 436513, 436518, 436526, 436536, 436538, C12Q 170, C12Q 168, C12Q 100, G01N 3353, G01N 33566, G01N 33563, G01N 33543, G01N 33533, G01N 33536, G01N 33537

Patent

active

052799360

ABSTRACT:
Methods are disclosed for separating a component of interest from a mixture containing the component of interest and other components. The method comprises contacting a first liquid medium containing the component of interest and other components with a second liquid medium that is of different density than and/or of different viscosity than the first liquid medium. The contact is carried out in such a way that mixing of the media is minimized or avoided. The component of interest is bound to magnetic particles. The contacted first liquid medium and second liquid medium are subjected to a magnetic field gradient to allow the magnetic particles to migrate into the second liquid medium and separation of the component of interest from other components is realized. Also disclosed are assays employing the present method. Kits for carrying out the present method and assays are also disclosed.

REFERENCES:
patent: 4731337 (1988-03-01), Luotola et al.
patent: 4777145 (1988-10-01), Luotola et al.
patent: 4868130 (1989-09-01), Hargreaves
patent: 4935147 (1990-06-01), Ullman et al.
Baldwin et al. (1987) BioMAT.TM.: A. Rapid, Self-Washing . . . Clin Chem 33: 1566-1567.
Freifelder (1983) Molecular Biology, Sci Book Int., pp. 88-91.
Beckman, Techniques in Centrifugation pp. 3, 10, 11.

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