Method of separating serum albumin and gamma-globulin from each

Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...

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210651, 210927, B01D 1300

Patent

active

043471382

ABSTRACT:
Serum albumin and a serum .gamma.-globulin are separated by filtration from each other in a solution of a mixture thereof by using an ultrafiltration membrane. The ultrafiltration membrane has a fractionation molecular weight of about 100,000. The total protein concentration and salt concentration in the mixture solution to be forced through the membrane are adjusted to not more than 4 g/dl and not more than 0.6 mole/l, respectively. Also, the pH of the solution is adjusted to a value of from about 3.8 to about 4.7.

REFERENCES:
patent: 3615024 (1971-10-01), Michaels
patent: 4222870 (1980-09-01), Sternberg et al.
Blatt, W. F. et al., "Solute Polarization and Cake Formation in Membrane Ultrafiltration . . . ", in Membrane Science and Technology, Plenum Press, N.Y. 1970.
Blatt, W. F., "Membrane Partition Chromatography . . . ", J. Agr. Food Chem., vol. 19, No. 4, 1971, pp. 589-594.
Porter, M. C. et al., "Membrane Ultrafiltration", Chemical Technology, Jan. 1971.

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