Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Patent
1985-09-11
1987-08-04
Kight, John
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
530364, 530380, 530386, 530389, 530395, 530420, 530421, 210656, 210905, 210927, 424101, C07G 700
Patent
active
046847235
ABSTRACT:
There is disclosed an improved method for separating and recovering proteins, particularly therapeutically active proteins, from an aqueous system also containing a component having the ability to create two liquid phases by use of salt partitioning technology. By the addition of water soluble inorganic salts to an aqueous system containing one or more therapeutically active proteins or nucleic acids, especially an aqueous system obtained from fractionation of a blood plasma fraction or from a tissue culture fluid resulting from a biotechnology production operation such as recombinant DNA and monoclonal antibody technologies, the aqueous system may be separated into two or more liquid phases. Such separated phases may be selectively enriched in components of the original aqueous system having differing solubility in the so-separated liquid phases.
REFERENCES:
patent: 3415804 (1968-12-01), Polson
patent: 3790552 (1974-02-01), Johnson et al.
patent: 4439358 (1984-03-01), Coan et al.
Dove George B.
Mitra Gautam
Johnson Lester E.
Kight John
Miles Laboratories Inc.
Nutter Nathan M.
Simonton Pamela A.
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