Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1989-10-03
1992-05-05
Raymond, Richard L.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530399, 530412, C07K 322
Patent
active
051109140
ABSTRACT:
A method for separating organic compounds, specifically proteins, is provided. The method for separating organic compounds is based on a combined pi-electron and electrostatic interaction of the exchanger with specific components of the organic compound. The method provides both an anionic and cationic exchanger. Each exchanger can provide more than one dimension of separation.
REFERENCES:
Awad, William M., Ph.D., M.D., [Abstract] "New General Techniques for Protein Separation", Innovation '89 Technology Transfer in Florida, presented by Gold Coast Venture Capital Club, University of Miami Venture Council Forum, Apr. 6, 1989.
Alpert et al. Preparation of a Porous Microparticulate Anion-Exchange Chromatography Support for Proteins, Journal of Chromatography, vol. 185, 1979, pp. 375-392.
Furuya et al. Electrostatic and hydrophobic effects in chromatography of Rabbit IgC Immunoglobulins on Aminohexyl Sepharose Substituted with bis(p-chlorophenyl)-acetic acid, Chemical Abstracts, vol. 100, No. 13, Mar. 26, 1984, p. 481, Abstract No. 101314u.
J. Lam, Separation and Protection of Gamma-electron-rich Compounds on Caffeine-impregnated Silica Gel Layers, Chemical Abstracts, vol. 80, No. 3, Jan. 21, 1974, p. 417, Abstract No. 14770q.
P. M. Reuben, Protein Chromatography Based upon Combined Pi-electron and Electrostatic Interactions, Chemical Abstracts, vol. 112, No. 21, May 21, 1990, p. 357, Abstract No. 194681x.
Kumar Shailendra
Raymond Richard L.
University of Miami
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