Method of separating or purifying proteins and other biopolymers

Details Method of separating or purifying proteins and other biopolymers Method of separating or purifying proteins and other biopolymers

1987-03-05

1989-10-10

Phillips, Delbert R.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530412, 530413, 530414, 530415, 530416, 530417, 530418, 530422, 530423, 530424, 530425, 530426, 435 68, 435 99, 435101, C07K 312, C07K 320, C07K 324, C12P 2100

Patent

active

048733172

ABSTRACT:
A method for the separation or purification of biopolymers comprises adsorbing the biopolymer on the surface of liquid oil droplets; and separating the adsorbed biopolymer from the oil droplets. The adsorbed biopolymer is separated from the oil droplets by mixing the droplets in an aqueous liquid, removing the lower aqueous phase and adding a fresh aqueous phase to the droplets, cooling the mixture to solidify and coalesce the oil and to cause it to release the adsorbed biopolymer to the fresh liquid, separating the fresh liquid and the biopolymer from the coalesced oil, separating the biopolymer from the fresh liquid.

REFERENCES:
patent: 4649110 (1987-03-01), Shilo et al.
patent: 4704360 (1987-11-01), Shoham et al.

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