Method of separating living cells

Details Method of separating living cells Method of separating living cells

1989-11-03

1991-07-09

Jones, W. Gary

Liquid purification or separation

Processes

Separating

210787, 210806, 43524025, 43524046, 436177, 494 37, B01D 2126

Patent

active

050303619

ABSTRACT:
A centrifugal separation method for living cells is disclosed in which a cell containing solution is charged in a rotor chamber confined by a steam pressure-resistant wall. A rotor having septa is disposed in the rotor chamber. The septa are rotatable in the same direction at the same angular velocity as the rotor while at least part thereof is immersed in a solution in the rotor when cells are to be centrifuged from the cell-containing solution. After the cells are separated and adhered to the inner surface of the rotor, a supernatant in the rotor is discharged from the rotor. Then a fresh cultivating medium is introduced into the rotor, and the septa and the rotor are rotated at mutually different angular velocities while at least part of the septa is immersed in the fresh medium in the rotor. As a result, the cells are again suspended in the fresh medium.

REFERENCES:
patent: 3168474 (1965-02-01), Stallman et al.
patent: 3536253 (1970-10-01), Anderson et al.
patent: 4939087 (1990-07-01), Van Wie et al.
Ishibashi, T. et al.; "New Oxygen Supplying Method for Animal Cell Reactors," Hitachi Review, vol. 4, (1987), pp. 13-17.

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