Method of separating embryo suspension mass

Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se

Reexamination Certificate

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C435S410000, C435S430100

Reexamination Certificate

active

07858371

ABSTRACT:
A method of separating an embryo suspension mass is provided. The method includes supplying an embryo suspension mass culture having a plurality of first particles of a first size and a plurality of second particles of a second size different at least in part from the first size of the first particles. The method also includes suspending the embryo suspension mass culture in a fluid to create a mixture and forcing the mixture through a filter while maintaining the mixture in the fluid to separate the first particles from the second particles.

REFERENCES:
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patent: 2005/0155119 (2005-07-01), Jayakumar
patent: 0899341 (1999-03-01), None
patent: 0899341 (1999-03-01), None
Tsukahara et al. “Separation and analysis of cell types involved in early stages of carrot somatic embryogenesis,” Plant Cell, Tissue and Organ Culture 47: 145-151, 1997.
Fujimura et al. “Synchronization of Somatic Embryogenesis in a Carrot Cell Suspension Culture,” Plant Physiol. (1979) 64, pp. 162-164.
Dunstan D I et al “Origins and Early Growth of Celery Embryoids”, New Phytologist, vol. 91, No. 1, 1982, pp. 121-128, XP002505675, ISSN: 0028-646X, Abstract p. 122.
Shohael et al: “Effect of Light on Oxidative Stress, Secondary Metabolites and Induction of Antixoidant Enzymes inEleutherococcus senticocosusSomatic Embryos in Bioreactor” Process Biochemistry, Elsevier, NL, vol. 41, No. 5, May 1, 2006, pp. 1179-1185, XP005350059, ISSN: 1359-5113, Point 2.2, p. 1180.
Peran-Quesada R et al: “Factors Affecting Maturation of Avocodo Somatic Embryos”, Scientia Horticulturae, Elsevier Science Publishers, XX, vol. 102, No. 1, Oct. 15, 2004, pp. 61-73, XP004560476, ISSN: 0304-4238, p. 62, LST Paragraph—p. 63, Paragraph 1.
Rodriguez D L et al: “Mechanical Purification of Torpedo Stage Somatic Embryos ofDaucus-carota L”, Plant Cell Tissue and Organ Culture, vol. 23, No. 1, 1990, pp. 9-14, XP008099086, ISSN: 0167-6857.
Rodriguez, D. L., et al., Mechanical purification of torpedo stage somatic embryos ofDaucus carota L.Plant Cell Tissue and Organ Culture, 1990, vol. 23, No. 1, pp. 9 to 14.
Peran-Quesada, P., et al., Factors affecting maturation of avocado somatic embryos, Scientia Horticulturale, 2004, vol. 102, No. 1, pp. 61 to 73; cf p. 62, the last paragraph, to p. 63, the first paragraph.
Shohael, A.M., et al., Effect of light on oxidative stress, secondary metabolites and induction of antioxidant enzymes ineleutherococcus senticcosissomatic embryos in bioreactor, Process Biochemistry, 2006, vol. 41, No. 5, pp. 1179 to 1185; cf p. 11 80 Item 2.2.
Dunstant, D.I., et al., Origin and early growth of celery embryoids. New Phytologist, 1982, vol. 91, No. 1, pp. 121 to 128; cf. the Abstract, p. 122, paragraph Embryoid plating, p. 126, paragraph 4.

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