Method of separating cells from a sample

Chemistry: analytical and immunological testing – Including sample preparation – Liberation or purification of sample or separation of...

Reexamination Certificate

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C436S518000, C436S528000, C436S174000, C436S824000, C435S007100, C435S007240, C435S287100, C435S287200, C435S288100

Reexamination Certificate

active

09756590

ABSTRACT:
Rare cells are separated from a sample fluid by a positive selection or negative selection antibody by centrifuging in a tube containing a harvesting float. The harvesting float has an axial passage and a density to settle in the sample fluid and expand the layer of the target component. The antibody is preferably coupled to a particulate carrier, such as a microbead, to attach either the target component or a contaminating component to the particulate carrier. In the positive separation, the particulate carrier is recovered in the axial passage of the float.

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