Method of separating albumin from serum by ion-exchange chromato

Details Method of separating albumin from serum by ion-exchange chromato Method of separating albumin from serum by ion-exchange chromato

1998-03-10

1999-12-14

Tsang, Cecilia

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Albumin

530363, 530412, 530416, 530417, C07K 14765

Patent

active

060019743

ABSTRACT:
A method of separating albumin from serum by ion exchange chromatography with membrane adsorbers characterized by high productivity and yields of high purity albumin. The separation of the albumin is carried out on highly basic anion exchange membranes and on highly acidic cation exchange membranes. The albumin fraction can be eluted from the anion exchange membrane such that it can be fed directly to the cation exchange membrane without any special conditioning and the albumin can be extracted therefrom as an end product.

REFERENCES:
patent: 4086222 (1978-04-01), Lindquist et al.
patent: 5728553 (1998-03-01), Goodey et al.
Freitag et al, J. Chromatogr. A., vol. 728, issue 1-2, pp. 129-137, 1986.

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