Method of separating activated human protein C

Details Method of separating activated human protein C Method of separating activated human protein C

1992-01-03

1993-03-02

Stoll, Robert L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

435219, 435815, 436548, C12Q 156

Patent

active

H00011487

ABSTRACT:
A method of separating activated human protein C, which comprises bringing a mixture containing activated human protein C having a gamma-carboxyglutamic acid (Gla) domain in the presence of a calcium ion into contact with a fixed antibody comprising an insoluble carrier and fixed thereto an antibody to a complex of human protein C and a calcium ion bound to the Gla domain whereby the activated human protein C is captured by the fixed antibody in the form in which the calcium ion is bound to the Gla domain. A method is also provided for separating activated human protein C from a mixture containing human protein C and activated human protein C, which comprises bringing a first mixture containing human protein C and activated human protein C having a Gla domain into contact with a first fixed antibody comprising an insoluble carrier and fixed thereto a first antibody to human protein C but not to activated human protein C to form a second mixture substantially free from human protein C while the human protein C is captured by the first fixed antibody, and bringing the second mixture in the presence of a calcium ion into contact with a second fixed antibody comprising an insoluble carrier and fixed thereto a second antibody to a complex of human protein C and a calcium ion bound at the Gla domain, whereby the activated human protein C is captured by the second fixed antibody in the form in which the calcium ion is bound to the Gla domain.

REFERENCES:
patent: 4902614 (1990-02-01), Wakabayashi et al.
Kisiel, W. et al. 1976, 15 4893-4900.
FEBS Letters, vol. 191, No. 1, Oct. 1985, pp. 75-81, M. Laurell et al., "Characterization of Monoclonal Antibodies . . . ".
Journal of Biological Chemistry, vol. 262, No. 28, Oct. 5, 1987, pp. 13798-13804, K. Ohlin et al. "Calcium-Dependent interaction . . . ".

Affiliated with

Also associated with

Rating

Method of separating activated human protein C does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of separating activated human protein C, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of separating activated human protein C will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-118969