Method of separating a peptide from a resin

Details Method of separating a peptide from a resin Method of separating a peptide from a resin

1991-04-12

1993-04-06

Lee, Lester L.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Separation or purification

530334, 525 5411, A61K 3702, C08F28300

Patent

active

052005070

ABSTRACT:
In solid phase peptide synthesis, resin-bound peptide is treated with hydrogen fluoride so as to cleave the peptide from the resin and form a first mixture of peptide, hydrogen fluoride and resin. The resin is removed from the first mixture so as to provide a resin-free second mixture including the polypeptide and the hydrogen fluoride. The polypeptide of the second mixture then is separated from the hydrogen fluoride.

REFERENCES:
patent: 4130514 (1978-12-01), Enkoji et al.
patent: 4824937 (1989-04-01), Deghenghi et al.
Nomizu et al., "Two-step hard acid deprotection/cleavage procedure for solid phase peptide synthesis". Int. J. Peptide Protein Res. 37, 1991 pp. 145-152.
Steward et al., Solid Phase Peptide Synthesis, 2nd Edition, Pierce Chemical Co., pp. 1-8, 38-40, 78-83, 89, 125-127 (1984).
Balasubramaniam et al., Synthesis of Neuropeptide Y, Int. J. Peptide Res., vol. 29, pp. 78-83 (1987).
Balasubramaniam et al., Syntheses and Receptor Affinities of Partial Sequences of Peptide YY (PYY), Peptide Research, vol. 1, pp. 32-35 (1988).
Barany et al., The Peptides, vol. 2, Eds. Gross and Meienhofer, Academic Press, Inc., pp. 65-77 (1980).
Horiki et al., Synthesis of the Merrifield Resin Esters of N-Protected Amino Acids with the Aid of Hydrogen Bonding, Chemistry Letters, pp. 165-168.
Wang et al., Enhancement of Peptide Coupling reactions by 4-Dimethylaminopyridine, Int. J. Peptide Res., vol. 18, pp. 459-467 (1981).
Multiple Peptide Systems advertisement (undated).

Affiliated with

Also associated with

Rating

Method of separating a peptide from a resin does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of separating a peptide from a resin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of separating a peptide from a resin will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-537706