Chemistry: analytical and immunological testing – Oxygen containing – Molecular oxygen
Patent
1993-10-14
1995-10-31
Snay, Jeffrey R.
Chemistry: analytical and immunological testing
Oxygen containing
Molecular oxygen
436138, 436172, 2504591, G01N 2164
Patent
active
054628792
ABSTRACT:
The present invention relates to a sensing instrument and method for measuring the concentration of an analyte. The sensing instrument includes: a sensing element including at least a first emissive indicator characterized by a bimolecular quenching rate constant k.sub.q, one or more fluorescence lifetimes .tau..sub.o above a lowest lifetime .tau..sub.oL, and capable of emitting analyte concentration dependent signals when exposed to an excitation signal in the presence of quencher; an excitation system which provides an amplitude modulated excitation signal at one or more radial modulation frequencies .omega.; a detector; and a processor. The processor univariantly processes the detected signals and provides output signals representative of analyte concentration. The sensing element and/or excitation system are configured to enable the instrument to operate sufficiently within the condition [(k.sub.q [Q]).sup.2 +.omega..sup.2 ].tau..sub.o.sup.2 >>1+2k.sub.q .tau..sub.o [Q] such that a slope of the relationship between the concentration dependent parameter and analyte concentration is independent of .tau..sub.o variability and/or heterogeneity for all analyte concentrations within the operating range and for all lifetimes .tau. .sub.o greater than .tau..sub.oL.
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Griswold Gary L.
Kirn Walter N.
Minnesota Mining and Manufacturing Company
Snay Jeffrey R.
Ubel F. Andrew
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