Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-04-05
1998-07-14
Ulm, John
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, 4353201, 435325, 435243, 530300, 530399, C12N 1510, C12N 1512, C07K 14435
Patent
active
057802793
ABSTRACT:
A method for identifying and selecting novel substrates for enzymes is provided. The method comprises constructing a gene fusion comprising DNA encoding a polypeptide fused to DNA encoding a substrate peptide, which in turn is fused to DNA encoding at least a portion of a phage coat protein. The DNA encoding the substrate peptide is mutated at one or more codons thereby generating a family of mutants. The fusion protein is expressed on the surface of a phagemid particle and subjected to chemical or enzymatic modification of the substrate peptide. Those phagemid particles which have been modified are then separated from those that have not.
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Matthews David J.
Wells James A.
Zoller Mark J.
Genentech Inc.
Saoud Christine
Schwartz Timothy R.
Ulm John
Winter Daryl B.
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