Method of selection of allelic exchange mutants

Details Method of selection of allelic exchange mutants Method of selection of allelic exchange mutants

1996-06-11

1998-12-01

Elliott, George C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4351723, 4353201, C12N 1500, C12N 1574, C12Q 168

Patent

active

058436642

ABSTRACT:
A process for replacing a nucleotide sequence in the genome of a mycobacterium strain comprises the steps of:

REFERENCES:
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Kalpana, G.V. et al., "Insertional Mutagenesis and Illegitimate Recombination In Mycobacteria," Proc. Nat'l. Acad. Sci. USA, vol. 88, pp. 5433-5437 (Jun. 1991).
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Sander, P. et al., "rpsL: A Dominant Selectable Marker For Gene Replacement In Mycobacteria," Mol. Microbiol., vol. 16, No. 5, pp. 991-1000 (1995).
Reyrat, J.M. et al., "The Urease Locus of Mycobacterium Tuberculosis and Its Utilization for the Demonstration of Allelic Exchange in Mycobacterium Bovis Bacillus Calmette-Guerin,"Proc. Nat'l. Acad. Sci. USA, vol. 92, pp. 8768-8772 (Sep. 1995).
Norman, E. et al., "Gene Replacement By Homologous Recombination In Mycobacterium Bovis BCG," Mol. Microbiol., vol. 16, No. 4, pp. 755-760 (1995).
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Balasubramanian, V. et al., "Allelic Exchange in Mycobacterium Tuberculosis With Long Linear Recombination Substrates," J. Bacteriol., vol. 1978, No. 1, pp. 273-279 (Jan. 1996).
Aldovini, A. et al., "The uraA Locus and Homologous Recombination In Mycobacterium Bovis BCG," J. Bacteriol., vol. 175, No. 22, pp. 7282-7289 (Nov. 1993).

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