Method of selection of allelic exchange mutants

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

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4352523, 4352531, C12N 121, C12N 1500

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060965495

ABSTRACT:
A process for replacing a nucleotide sequence in the genome of a mycobacterium strain comprises the steps of:

REFERENCES:
patent: 5807723 (1998-09-01), Aldovini et al.
Quandt, J. et al., "Versatile Suicide Vectors Which Allow Direct Selection for Gene Replacement in Gram-Negative Bacteria," Gene, pp. 15-21 (1993).
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Pelicic, V. et al., "Expression of the Bacillus Subtilis sacB Gene Confers Sucrose Sensitivity On Mycobacteria," J. Bacteriol., vol. 178, No. 4, pp. 1197-1199 (Feb. 1996).
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Norman, E. et al., "Gene Replacement by Homologous Recombination In Mycobacterium Bovis BCG," Mol. Microbiol., vol. 16, No. 4, pp. 755-760 (1995).
Marklund, B.I. et al., "Gene Replacement Through Homologous Recombination In Mycobacterium Intracellular," J. Bacteriol., vol. 177, No. 21, pp. 6100-6105 (Nov. 1995).
Husson, R. et al., "Gene Replacement and Expression of Foreign DNA in Mycobacteria," J. Bacteriol., vol. 172, No. 2, pp. 519-524 (Feb. 1990).
Balasubramanian, V. et al., "Allelic Exchange in Mycobacterium Tuberculosis With Long Linear Recombination Substrates," J. Bacteriol., vol. 178, No. 1, pp. 273-279 (Jan. 1996).
Aldovini, A. et al., "The uraA Locus and Homologous Recombination In Mycobacterium Bovis BCG," J. Bacteriol., vol. 175, No. 22, pp. 7282-7289 (Nov. 1993).
Guilhot et al. (1994) Efficient transposition in mycobcteria: construction of Mycobacterium smegmatis insertional mutant libraries. J. Bacteriol. 176:535-539, Jan. 1994.

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