Method of selecting recombinant DNA-containing streptomyces

Details Method of selecting recombinant DNA-containing streptomyces Method of selecting recombinant DNA-containing streptomyces

1992-12-02

1995-03-14

Martinell, James

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352523, 43525235, 4353201, 536 231, 536 232, 536 237, C12N 1500, C12N 1511

Patent

active

RE0348759

ABSTRACT:
A novel method of selecting Streptomyces recombinant DNA-containing host cells and vectors useful in the method are described. The vectors confer tylosin resistance to sensitive Streptomyces host cells and thus provide a convenient method of selecting Streptomyces transformants. The novel tylosin resistance-conferring gene described can be isolated on an .about.2.6 kb KpnI restriction fragment from plasmid pSVB2. Plasmid pSVB2 can be isolated from Streptomyces lividans TK23/pSVB2 (NRRL 15880).

REFERENCES:
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patent: 4468462 (1984-08-01), Malin et al.
Benveniste and Davies, 1973, Proceedings of the National Academy of Sciences, USA 70(8):2276.
Thompson et al., 1980, Nature 286:525.
Thompson et al., 1982, Gene 20:51.
Katz et al., J. Gen. Microbiology, 129:2703 (1983).

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