Method of selecting mammalian cell lines having improved product

Details Method of selecting mammalian cell lines having improved product Method of selecting mammalian cell lines having improved product

1994-11-03

1997-03-18

Elliott, George C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 4353201, 435325, 435366, 435369, C12N 506, C12N 1563, C12P 2106, C12Q 168

Patent

active

056122136

ABSTRACT:
A method of selecting transfected cell lines for heterologous protein expression from a population of cells some of which include an endogenous sequence for an amplificable marker and some of which do not include the sequence. The method is based on a minimum amplifiability index which, contrary to current practice, focuses cell lines having relatively low productivity prior to amplification. Transfected cell lines selected with the instant invention produce greater amounts of heterologous protein than cells selected according to the prior art. In one embodiment, the amplifiability index is used to select for further amplification a cell line transfected with a vector containing the coding sequence for human Factor VIII.

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