Method of selecting Escherichia coli strain which highly...

Details Method of selecting Escherichia coli strain which highly... Method of selecting Escherichia coli strain which highly...

2003-12-11

2009-02-24

Prouty, Rebecca E (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S034000, C435S106000, C435S183000, C435S069100, C536S023200

Reexamination Certificate

active

07494801

ABSTRACT:
The invention relates to a selection method of anEscherichia colimutant strain which expresses an exogenous gene at a high level, wherein stress resistance such as hydrogen peroxide decomposition activity is used as an index, theEscherichia colimutant strain selected thereby and production methods of enzyme using the strain and of useful compounds like amino acids (especially L-amino acid) and the like using the strain. According to the selection method of the invention, anEscherichia colimutant strain where gene expression amount does not decrease with passage can be obtained, and a compound using a plant-derived ammonia lyase can be efficiently produced.

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