Method of searching for gene encoding nuclear transport protein

Details Method of searching for gene encoding nuclear transport protein Method of searching for gene encoding nuclear transport protein

2007-03-27

2007-03-27

Strzelecka, Teresa E. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S007100, C435S091200, C435S320100, C530S350000, C536S023400

Reexamination Certificate

active

10204310

ABSTRACT:
Each gene in a group comprising many genes is cloned into an expression vector so that its gene product should be expressed as a fusion protein with a protein emitting self-fluorescence to construct a gene expression library. The gene expression library is introduced into cells of two groups to express the fusion proteins in the cells, and only one cell group is stimulated by a drug or the like. Then, cells in which the fusion proteins are localized in the nuclei or their nuclei are isolated from cells of the two groups, respectively. By comparing these cells, genes coding for proteins that are transported into the nuclei specifically in response to a certain stimulus when the stimulus is applied to the cells are retrieved.

REFERENCES:
patent: 5891924 (1999-04-01), Aggarwal
patent: WO 00/17643 (2000-03-01), None
patent: WO 00/23615 (2006-04-01), None
Sutherland, H.G.E. et al., “Large-scale identification of mammalian proteins localized to nuclear sub-compartments”, Hum. Mol. Gen., vol. 10, pp. 1995-2011 (Sep. 2001).
Tate, P. et al., “Capturing novel mouse genes encoding chromosomal and other nuclear proteins”, J. Cell Sci., vol. 111, pp. 2575-2585 (1998).
Sawin K. E. et al. “Identification of fission yeast nuclear markers using random polypeptide fusions with green fluorescent protein”, PNAS, vol. 94, pp. 15146-15151 (1996).
Rakowicz-Szulczynska, E.M., et al., “Nuclear uptake of monoclonal antibody to a surface glycoprotein and its efect on transcription”, Arch. Biochem. Biophys., vol. 271(2), p. 366-79 (1989).
Rakowicz-Szulczynska, E.M., et al., “intracellular receptor binding and nuclear transport of nerve growth factor in intact cells and a cell-free system”, Mol. Carcinog., vol. 2, pp. 47-58 (1989).
Gonzales, C. et al., “Protein traps: using intracellular localization for cloning”, Trends in Cell Biol., vol. 10, pp. 162-165 (Apr. 2000).
Turpin, P. et al., “Nuclear transport and transcriptional regulation”, FEBS Letters, vol. 452, pp. 82-86 (1999).
M.M. Rolls et al.: “A visual screen of a GFP-fusion library identifies of a new type of nuclear envelope membrane protein” J. Cell. Biol., vol. 146, No. 1, pp. 29-43 1999.
European Office Action dated Nov. 20, 2006, Issued I corresponding European patent Application No. 01979034.4.
A. Beg, et al., “IxB interacts With The Nuclear Localization Sequences of The Subunits of NF-xB: A Mechanism For Cytoplasmic Retention”, Genes and Development, vol. 6, 1992, pp. 1889-1913.

Affiliated with

Also associated with

Rating

Method of searching for gene encoding nuclear transport protein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of searching for gene encoding nuclear transport protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of searching for gene encoding nuclear transport protein will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3780237