Method of screening to find new antibiotics

Details Method of screening to find new antibiotics Method of screening to find new antibiotics

1998-01-15

1999-09-28

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168

Patent

active

059586958

ABSTRACT:
An easily automated method of screening for antibiotics active against erythromycin resistant strains, comprising footprinting an antibiotic on the domain II of 23S rRNA, isolating rRNA by incubating an antibiotic with ribosomes, modifying ribosomes with a chemical modifying agent, isolating modified rRNA and subjecting it to the reverse transcriptase-mediated primer extension and gel electrophoresis on a DNA sequencer to determine the extent of antibiotic-induced protection of an rRNA nucleotide from chemical modification.

REFERENCES:
Gu et al., PNAS, vol. 91, 1994, pp. 5612-5616, Jun. 1994.

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