Method of screening oxysterol activation of LXRα

Details Method of screening oxysterol activation of LXRα Method of screening oxysterol activation of LXRα

2005-05-17

2005-05-17

Pak, Michael (Department: 1646)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007100, C435S007200, C435S011000, C435S069100

Reexamination Certificate

active

06893830

ABSTRACT:
The present invention provides a method of screening for agonists of an oxycholesterol activator of LXRα transcription, comprising the steps of: introducing a reporter construct and an LXR expression construct into a host cell; treating the host cell with potential LXR-specific ligands; and identifying compounds which activate LXRα transcription. Also provided is a method of screening for antagonists of an oxycholesterol activator of LXRα transcription, comprising the steps of: introducing a reporter construct and an LXR expression construct into a host cell; pretreating the host cell with activators of LXRα transcription; contacting the host cell with potential antagonists of LXRα transcription; and identifying compounds which block the activation the LXRα transcription.

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