Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or...
Patent
1997-09-11
1999-03-23
Saucier, Sandra E.
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
435 29, 435375, A01N 102, C12Q 102, C12N 500
Patent
active
058857664
DESCRIPTION:
BRIEF SUMMARY
The subject of the invention is a process for testing a substance, the said substance possibly being active in the hair field.
Hair field is understood to mean everything which can relate to the hair of an individual. Thus, "substance which is possibly active in the hair field", or simply "substance" subsequently in the text, is understood to mean any molecule or collection of molecules exhibiting a potential activity in the hair field and in particular any molecule or collection of molecules potentially having an activity on the colouring, the survival, the slowing down or halting of the growth, the loss or alternatively the intensified growth of hair follicles. The substance to be tested can be used according to the process of the invention either in its molecular form or in the form of a composition containing the molecule to be tested.
To date, the prior art mentions two main methods for testing for a substance which is possibly active in the hair field. The first consists in carrying out tests on volunteers and observing more or less rapidly the effects of the tested substance. It is obvious that this method has many disadvantages, including in particular that of being applied to human beings, which obviously limits, for ethical reasons, the field of application of the method. Thus, the number and the quality of the substances tested is limited. Moreover, these tests are generally cumbersome to carry out and extend over lengthy time periods. The results of the test are then in the majority of cases observations of phenotypic modifications of the hair follicle.
The second method known in the prior art is that described in Patent EP 434,319. This method consists in carrying out the tests according to a process which involves four stages, including in particular a stage of isolation of a viable hair follicle which has retained an intact bulb. To do this, it proves necessary to carry out a microdissection of the hair follicle, from a sample withdrawn from a subject. The latter in any case retains a mark of this operation. This limits the supply of hair follicles and the implementation of the test method is cumbersome and lengthy.
After much work, the Applicant Company has been able to show that it is possible to use, in the hair field, at least one plucked hair follicle for testing a substance which is possibly active in the hair field.
The subject of the invention is therefore a process for testing a substance which is possibly active in the hair field, characterized in that at least one hair follicle which has been plucked from a subject is incubated in a suitable culture medium for a sufficient time, in that this plucked hair follicle is brought into contact with a substance which is possibly active in the hair field, in that a label of the activity of the said tested substance is quantitatively determined and in that the results of the quantitative determination are evaluated with respect to a control.
The hair follicle used in the process according to the invention was isolated by plucking. The plucking consists of a sudden separation of the hair follicle and of the dermis, generally carried out by a more or less strong pull exerted on the hair shaft of the follicle.
The plucked hair follicle can be intact, that is to say contain all the parts recognized by the person skilled in the art as constituting it (see, Treatments!, Charles Zviak, published by Masson, 1987). Mention will be made, for example, and nonlimitingly, among these parts, of the dermal papillae, the hair bulb, the epithelial sheaths and the sebaceous gland. However, of course, the invention is not limited to the intact plucked hair follicle and also relates to any plucked hair follicle which, after isolation, would have retained only a portion of its constituent parts.
Plucking in order to isolate the hair follicle is a particularly advantageous procedure since it has the advantages of being noninvasive and therefore nontraumatizing for the subject and of being simple and fast to carry out.
An additional advantage lies in the fact tha
REFERENCES:
patent: 5229271 (1993-07-01), Philpott
patent: 5279969 (1994-01-01), Lavrer et al.
patent: 5712169 (1998-01-01), Bernard et al.
Brannan PG et al., J. Lipid Res. 16: 7-11 (1975).
Hoffmann R et al., J. Invest. Dermatology 103(4): 530-33 (1994).
Arase S et al., J. Dermatol. Sci 2(5):353-360 (1991).
FASEB Journal, vol. 6, No. 3, 1992 Washington, D.C., pp. 911-913, J.J. Jimenez et al, Interleukin 1 protects hair follicles . . . , p. 911, col. 1, lines 1-9, p. 912, col. 1, lines 13-28.
Billoni Nelly
Mahe Yann
Michelet Jean-Fran.cedilla.ois
Saucier Sandra E.
Societe l'Oreal S.A.
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