Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2011-07-12
2011-07-12
Paras, Jr., Peter (Department: 1632)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S041000, C435S071100, C435S320100
Reexamination Certificate
active
07977097
ABSTRACT:
A method of screening multiply transformed/transfected cells to identify those cells expressing at least two peptides or proteins of interest. The method comprising: 1. Simultaneously or sequentially transforming a cell with at least two different expression cassettes in which the gene of interest is linked via an IRES to a fluorescent marker gene. Each marker gene is different. 2. Providing conditions in which expression of the genes will occur. 3. Identifying cells expressing proteins by detecting the different fluorescent signals.
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Gay Robert D.
Gray Peter Philip
Sunstrom Noelle-Anne
Halstead David P.
Montanari David
Paras, Jr. Peter
Ropes & Gray LLP
The University of New South Wales
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