Method of screening for agents that regulate the shedding of...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S015000

Reexamination Certificate

active

10471122

ABSTRACT:
The present invention provides a method of screening for agents which may regulate or inhibit the activities of TACE and TGF-α. These agents may act by enhancing or inhibiting the activity of DGK-δ. An activity of DGK-δ is the enzymatic conversion of DAG to PA. The method includes contacting a cell or organism with a test agent determining the activity of DGK-δ within the cell. An agent with reduces or increases the activity of DGK may be used to regulate or inhibit the activity of TACE and TGF-α. Such agents can be used to treat diseases such as cancer where cell growth and division and inflamation are important factors.

REFERENCES:
Sakane et al., J.B.C., 271(14), 8394-8401, 1996.
Zhao, Jinsong, “Pulmonary Hypoplasia in Mice Lacking Tumor Necrosis Factor-α Converting Enzyme Indicates an Indispensable Role for Cell Surface Protein Shedding during Embryonic Lung Branching Morphogensis.” Development Biology, 232, 204-218 (2001).

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