Method of screening apoptosis accelerating compound or...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007200

Reexamination Certificate

active

07985556

ABSTRACT:
Screening methods for determining pro-apoptotic compounds or anti-apoptotic compounds comprise measuring the interaction between p53 and NEDL1 in the presence and in the absence of a test compound, and comparing the strength of interaction between p53 and NEDL1 in the presence and in the absence of the test compound.

REFERENCES:
patent: 02/090549 (2002-11-01), None
patent: 03/012102 (2003-02-01), None
patent: 03/018842 (2003-03-01), None
Kou Miyazaki, et al., “NEDL1, a Novel Ubiquitin-protein Isopeptide Ligase for Dishevelled-1, Targets Mutant Superoxide Dismutase-1”, The Journal of Biological Chemistry, 2004, pp. 11327 to 11335, vol. 279, No. 12.
Yili Yang, et al., “Small molecule inhibitors of HDM2 ubiquitin ligase activity stabilize and activate p53 in cells”, Cancer Cell, Jun. 2005, pp. 547 to 559, vol. 7, No. 6.
Kou Miyazaki, et al., “A novel HECT-type E3 ubiquitin ligase, NEDL2, stabilizes p73 and enhances its transcriptional activity”, Biochemical and Biophysical Research Communication, 2003, pp. 106 to 113, vol. 308, No. 1.
Daniel R. Rosen, et al., “Mutations in Cu/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis”, Letters to Nature, Mar. 1993, pp. 59 to 62, vol. 362, No. 3721.

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