Method of sandwich immunoassay for N-peptide

Details Method of sandwich immunoassay for N-peptide Method of sandwich immunoassay for N-peptide

1995-12-08

1997-12-30

Chin, Christopher L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 792, 435 795, 435 78, 435 79, 435176, 435960, 436518, 436542, 436547, 436548, 436811, G01N 3353, G01N 33543

Patent

active

057029105

ABSTRACT:
The present invention relates to a sandwich immunoassay for rapidly and readily measuring N-peptide using two kinds of monoclonal antibodies recognizing different portions of the N-peptide. The method for measuring N-peptide or a precursor thereof includes the steps of: incubating a mixture containing a sample and a first monoclonal antibody recognizing a portion of N-peptide; adding a labelled second monoclonal antibody recognizing a portion of N-peptide to the mixture, followed by further incubation; and detecting the resulting antigen-antibody complex in the mixture. Alternatively, the method includes the steps of: incubating a mixture containing a sample, a first monoclonal antibody recognizing a portion of N-peptide, and a labelled second monoclonal antibody recognizing another portion of N-peptide; and detecting the resulting antigen-antibody complex.

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