Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-04-17
2000-11-07
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4352871, 4352872, 4352883, 4352887, 436164, 436172, 436518, 436524, 436527, 436531, 436805, 436809, 422 58, G01N 33543, G01N 3368
Patent
active
061434965
ABSTRACT:
Methods of detecting and quantifying genomic nucleic acid molecule sequences are provided using the simultaneous amplification of a plurality of discrete nanoliter-sized samples. A miniaturized closed assembly is also provided for carrying out amplification of a nucleic acid molecule by polymerase chain reaction in multiple nanoliter-sized samples. Methods of filling miniaturized sample chambers are also provided as are methods for determining the number of template molecules in a sample by conducting replicate nucleic acid sequence amplification reactions on a set of terminally diluted samples and counting the number of positive amplification reactions. The methods can be used to detect a single starting nucleic acid target molecule.
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Brown James F.
Kalinina Olga V.
Silver Jonathan E.
Chin Christopher L.
Cytonix Corporation
The United States of America as represented by the Department of
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