Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2007-10-09
2007-10-09
Martinell, James (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S006120
Reexamination Certificate
active
10470846
ABSTRACT:
The present invention relates to a method of disrupting a conjugate comprising a biotin compound and a biotin-binding compound, said method comprising the step of contacting said conjugate with an effective amount of a substantially aqueous solution under conditions such that the conjugate is disrupted, preferably reversibly disrupted, thereby forming a biotin compound and a biotin-binding compound. In particular, the invention relates to a method of reversibly releasing a biotinylated moiety from a streptavidin (or avidin) coated support. Uses of the methods in techniques of detection, identification, determination, purification, separation and/or isolation of target proteins or nucleic acid molecules are also encompassed by the present invention.
REFERENCES:
patent: 5395856 (1995-03-01), Haase
patent: 6022688 (2000-02-01), Jurinke et al.
patent: 6303309 (2001-10-01), Jurinke et al.
patent: 0 456 304 (1991-11-01), None
patent: WO97/00329 (1997-01-01), None
patent: WO 00/70073 (2000-11-01), None
patent: WO 01/51067 (2001-07-01), None
Tong et al., “Solid-Phase Method for the Purification of DNA Sequencing Reactions ,”Anal. Chem.1992, pp. 2672-2677, vol. 64. American Chemical Society.
Ding et al., “Temperature Control of Biotin Binding and Release with A Streptavidin-Poly(N-isopropylacrylamide) Site-Specific Conjugate, ”Bioconjugate Chemistry, 1999, pp. 395-400, vol. 10. American Chemical Society.
Morris et al., “Affinity Precipitation of Proteins by Polyligands,”Biotechnology and Bioengineering, 1993, pp. 991-997, vol. 41, John Wiley & Sons, Inc.
Garret-Flaudy, et al., “Use of the Avidin (Imino)biotin System as a General Approach to Affinity Precipitation,”Biotechnology and Bioengineering, 2001, pp. 223-234, vol. 71. John Wiley & Sons, Inc.
Foulon et al., “Preparation and Biological Evaluation of an Astatine-211 Labeled Blotin Conjugate: Biotinyl-3-[211At]astatoanilide,”Nuclear Medicine and Biology, 1997, pp. 135-143, vol. 24. Elsevier Science Inc.
Green et al., “The Kinetics of Biotin Binding-The Affinity for Avidin,”Biomolecular Engineering, May 2000, p. 170, vol. 16. Abstract XP-002226761.
Magnetic Biosolutions AB
Martinell James
Rothwell Figg Ernst & Manbeck
LandOfFree
Method of reversibly disrupting a conjugate comprising a... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of reversibly disrupting a conjugate comprising a..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of reversibly disrupting a conjugate comprising a... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3868025