Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2011-08-16
2011-08-16
Yang, Nelson (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007200, C435S968000, C436S519000, C436S172000, C530S391300, C530S391500
Reexamination Certificate
active
07998694
ABSTRACT:
The present invention relates to a method of revealing a biological process using a FRET measurement, which comprises the following steps:incorporating, into a measurement medium containing a lipid membrane, a biological entity X coupled with a first member of a pair of FRET partners and a second biological entity Y coupled with the second member of the pair of FRET partners, the energy-donating member of the pair of FRET partners having a long lifetime and the members of said pair of FRET partners being located on either side of the lipid membrane;exciting the measurement medium at the excitation wavelength of the energy-donating member; andmeasuring the FRET signal or the variations in said signal emitted in said culture medium.
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Ansanay Hervé
Mathis Gerard
Trinquet Eric
Cis Bio International
Millen White Zelano & Branigan P.C.
Yang Nelson
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