Method of removing N-terminal amino acid residues from eucaryoti

Details Method of removing N-terminal amino acid residues from eucaryoti Method of removing N-terminal amino acid residues from eucaryoti

1995-03-08

1998-06-09

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 681, 435172212, C12P 2102, C12N 948, C12N 1501, C12N 1509

Patent

active

057632154

ABSTRACT:
A method of sequentially removing one or more N-terminal amino acid residues from an analog of a eucaryotic polypeptide synthesized in a foreign host, comprises contacting the eucaryotic polypeptide analog with aminopeptidase under suitable conditions permitting sequential removal of N-terminal amino acid residues, where the polypeptide analog contains an amino acid residue or sequence of residues which blocks the action of an aminopeptidase located at a position other than the N-terminal end of the polypeptide analog. A specific embodiment of the invention concerns removing both an N-terminal methionine residue and its adjacent Leucine residue from a growth hormone analog produced in bacteria by contacting the growth hormone analog with Aeromonas aminopeptidase under suitable conditions permitting removal of the N-terminal methionine and Leucine residues. The invention also concerns eucaryotic polypeptide analogs produced in accordance with the methods of the invention.

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Seeburg et al DNA (1983) 2(1) PP. 37-45.
McLean et al J. Biol. Chem. (1984) 259(10) 6498-504.

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