Method of removing lipid inhibitors from limulus amebocyte lysat

Details Method of removing lipid inhibitors from limulus amebocyte lysat Method of removing lipid inhibitors from limulus amebocyte lysat

1979-11-13

1981-07-21

Gron, Teddy S.

Compositions

Nonreactive analytical, testing, or indicating compositions

23230B, 210634, 424 12, 424 95, 424101, 435 34, 435 38, 435259, G01N 3100, G01N 2104, G01N 3348

Patent

active

042797748

ABSTRACT:
The improved method of the present invention involves the removal of lipid inhibitors from Limulus amebocyte lysate to increase the sensitivity and quality of the lysate. The method comprises intimately contacting, as by mixing and stirring, Limulus amebocyte lysate with a selected binary liquid solvent system for a time sufficient to draw water into the solvent system from the lysate and to effectively extract and denature the lipid inhibitors in the lysate. The ability of the binary solvent to draw in water from the lysate and form, in effect, a new tertiary system is the important factor in this methodology. The amount of water drawn into the solvent is controlled by the amount of polar solvent in the solvent system. After this extraction and denaturation, the lysate is then separated from the solvent system and recovered in purified form of increased sensitivity.

REFERENCES:
patent: 2094931 (1937-10-01), Schultze
patent: 3915805 (1975-10-01), Levin
patent: 4038029 (1977-07-01), Teller et al.
patent: 4056468 (1977-11-01), Breiter et al.
patent: 4096091 (1978-06-01), Hopkins
patent: 4107077 (1978-08-01), Sullivan et al.

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