Method of regulating expression of a foreign gene by controlling

Details Method of regulating expression of a foreign gene by controlling Method of regulating expression of a foreign gene by controlling

1991-11-27

1994-06-21

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

4351723, 43525233, 4353201, C12N 988, C12N 1500, C12N 121, C12N 1563

Patent

active

053227867

ABSTRACT:
Escherichia coil carrying a hybrid plasmid having been constructed by inserting a desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene therein was cultured at a temperature 40.degree. C. or over so that the expression of said desired foreign gene was suppressed. This E. coil (i.e., transformant) was cultured at 40.degree. C. or over in a first process to suppress the expression of the foreign gene and to support sufficient cell growth and thereafter below 40.degree. C. in a second process to release the suppression of the expression so as to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture.

REFERENCES:
Gilbert et al, Journal of Bacterology, vol. 161, No. 1, pp. 314-320, Jan. 1985.
Anson et al., Gene, vol. 58, Nos. 2-3, pp. 189-199, 1987.
Mieschendal et al, Biotechnology, vol. 4, No. 9, pp. 802-808 1986.
Remaut et al. Gene, vol. 15, pp. 81-93, 1981.
Remaut et al, Nucelic Acids Research, vol. 11, No. 14, pp. 4677-4688, 1983.
Nichols et al, Methods in Enzymology, vol. 101, pp. 155-164, 1983.
de Boer et al, Proc. Natl. Sci. USA, vol. 80, pp. 21-25, 1983.
Amann et al, Gene, vo. 25, No. 2/3, pp. 167-178, Nov. 1983.

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