Method of regulating expression of a foreign gene by controlling

Details Method of regulating expression of a foreign gene by controlling Method of regulating expression of a foreign gene by controlling

1991-02-25

1992-08-18

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 435232, 43525233, 4353201, 935 29, 935 41, 935 42, C12N 121, C12N 988, C12N 1511, C12N 1560

Patent

active

051399350

ABSTRACT:
Escherichia coli carrying a hybrid plasmid having been constructed by inserting a desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene therein was cultured in a medium containing a sugar component utilizable by the E. coli as a carbon source at a concentration of 0.3% or more so that the expression of said desired foreign gene was suppressed. This E. coli (i.e., transformant) was cultured in the medium in which the sugar concentration was maintained at 0.3% or more in a first process so as to supress the suppression of the foreign gene and to support sufficient cell growth and thereafter at less than 0.3% in a second process to release the suppression of the expression so as to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture.

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