Method of regenerating plantlets from mesophyll protoplasts of p

Details Method of regenerating plantlets from mesophyll protoplasts of p Method of regenerating plantlets from mesophyll protoplasts of p

1988-01-19

1990-12-11

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524049, 43524051, 43524054, C12N 502, C12N 500

Patent

active

049770870

ABSTRACT:
A method for regenerating plantlets from protoplasts isolated from mesophyll cells of abacterial seedlings of red bean (Phaseolus angularis). Protoplasts cultured in certain liquid media could divide continuously and many protoplast-derived calli may be obtained. In addition, various growth media containing the optimum concentration and the type of sugar, mineral salts, vitamins, and combinations and concentrations of several phytohormones for protoplast growth, division, callus formation and plantlet regeneration are provided.

REFERENCES:
Crepy et al., 1986, Plant Cell Reports 5(2): 124-126.
Pelcher et al., 1974, Plant Science Letters 3(2): 107-111.
Xu et al., 1981, Z. Pfanzenphysiol, 104(4): 289-298.

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