Method of reducing background in biotin-based assays

Details Method of reducing background in biotin-based assays Method of reducing background in biotin-based assays

1995-08-16

2000-08-01

Housel, James C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 6, 435 71, 435 795, 435962, 436518, 436528, 436531, 436825, 436826, G01N 3353, G01N 33543, G01N 33544, C12Q 168

Patent

active

060965088

ABSTRACT:
This invention relates to an improvement in a method for detecting labeled molecules and especially biotinylated molecules and particularly relates to a method for reducing background signal problems in such detection methods.

REFERENCES:
patent: 3654090 (1972-04-01), Schuurs et al.
patent: 3839153 (1974-10-01), Schuurs et al.
patent: 4016043 (1977-04-01), Schuurs et al.
patent: 4152411 (1979-05-01), Schall, Jr.
patent: 4334017 (1982-06-01), Plotkin et al.
patent: 4684609 (1987-08-01), Hsu
patent: 4746604 (1988-05-01), Mowshowitz
patent: 4889798 (1989-12-01), Rabbani
patent: 4894325 (1990-01-01), Eaglehardt et al.
patent: 4994373 (1991-02-01), Stavrianopoulos et al.
patent: 5028525 (1991-07-01), Gray et al.
patent: 5252466 (1993-10-01), Cronan, Jr. et al.
patent: 5255325 (1993-10-01), Miller et al.
patent: 5262334 (1993-11-01), Berenson et al.
patent: 5281521 (1994-01-01), Trojanowski et al.
patent: 5332679 (1994-07-01), Simons et al.
patent: 5413906 (1995-05-01), Eberle et al.
patent: 5482698 (1996-01-01), Griffiths
patent: 5487975 (1996-01-01), Miller et al.
Duhamel et al., "Prevention of Nonspecific Binding of Avidin," Methods In Enzymology, 184:201-207 Academic Press Inc., 1990.
Woods et al., "Suppression of Endogenous Avidin Bending Activity in Tissues and Its Relevance to Biotin-Avidin Detection Systems," J. Histochem. Cytochem. 29(10):1196-1204, 1981.
International Search Report of PCT/US96/13046 dated Jul. 08, 1997.
Box V of the Written Opinion Dated Jun. 30, 1997 for PCT/US96/13046.
Annals of Clinical and Lab. Sci, 24(4):324-338 (1994)(with Abstract also) Szakacs et al.
Hofmann et al., "Avidin Binding of Carboxyl-Substituted Biotin and Analogues", Biochemistry 21:978-984 (1982).
Mock et al., "A Study of the Interaction of Avidin with 2-Anilinonaphthalene-6-Sulfonic Acid as a Probe of the Biotin Binding Site", Biochimico et Biophysica Acta 956:23-29 (1988).
Clark et al., "Suppression of Nonspecific Binding of Avidin-Biotin Complex (ABC) to Proteins Electroblotted to Nitrocellulose Paper", J. Histochemistry and Cytochemistry 34(11):1509-1512 (1986).
International Search Report of PCT/US96/13046 dated Oct. 24, 1996.
Hsu, Su-Ming, "Immunoperoxidase Techniques Using the Avidin-Biotin System",In: Enzyme-Mediated Immunoassay, 1985, pp. 467-476.

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