Method of recovering shiga-like toxins and vaccines comprising i

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

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4242341, 4242361, 4242351, 4242411, 435 737, 435 78, 536 53, 536124, A23J 100

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059228482

ABSTRACT:
Disclosed are methods of purifying shiga-like toxins (SLTs) from Polymyxin B sulfate extracts of Verotoxin-producing Escherichia coli. The methods are facile, efficient and reproducible. In another aspect, the toxin is inactivated for use in a vaccine against SLT mediated disease conditions.

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