Method of recovering peroxidase from seed hulls using a freeze-t

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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435189, 435190, 435814, 435816, C12N 908, C12N 906, C12N 904

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active

054910854

ABSTRACT:
Peroxidase can be recovered from seed hulls in an improved method using a freeze-thaw technique. First, the seed hulls are comminuted, placed into water and homogenized. Next, the homogenate is frozen then thawed. The enzyme is then recovered from the aqueous solution by conventional means. Soybean or rice seed hulls can be used in this process.

REFERENCES:
patent: 3947324 (1976-03-01), Lakshminarayanan
patent: 4228240 (1980-10-01), Dawson et al.
patent: 4698306 (1987-10-01), Noda et al.
Albara et al. J. of Biochem. 90. pp. 489-496. (1981).

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