Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.
Patent
1986-09-09
1987-08-25
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Glycoprotein, e.g., mucins, proteoglycans, etc.
424 85, 424 88, 435 68, 435 70, 4351722, 4351723, 530377, 530403, 530808, 530809, 530416, C07K 114, C07K 322, C07K 328, C07K 1300
Patent
active
046894012
ABSTRACT:
Substantially pure, intracellularly produced, soluble recombinant ricin toxin A (RTA) is recovered from transformed cells by disrupting the cell membrane, removing insoluble cell membrane materials from the disruptate, adjusting the pH of the cell membrane material-free solution to 6 to 6.5 and the conductivity to 1.25 to 1.75 millisiemens, passing the adjusted solution through a bed of SP-cellulose cation exchanger, and eluting the substantially pure RTA from the bed.
REFERENCES:
patent: 4340535 (1982-07-01), Voisin et al.
patent: 4414148 (1983-11-01), Jansen et al.
patent: 4590071 (1986-05-01), Scannon et al.
patent: 4614650 (1986-09-01), Jansen et al.
Proc-Natl. Acad. Sci., USA 77, No. 9 (1980), 5419-5422, Krolick et al.
Monoclonal Antibodies in Clinical Medicine, Academic Press Thorpe et al., 168-190 (1982).
Eur. J. Biochem., 116(1981), 447-454, Thorpe et al.
Toxicon, (1984), 22(2), 265-277, Cushley et al.
Analytical Biochemistry, 146, 206-210 (1985), Simmons et al.
Clinical Allergy, (1983), 13, 553-561, Davison et al.
Perspectives in Toxicology, Bernheimer Ed. 1977, pp. 122-147, Olsnes.
Europ. J. Biochem. 137, 57-65 (1983), Butterworth et al.
Molecular Action of Toxins & Viruses, Elsevier Biomedical Press, 1982, pp. 51-105, Olsnes et al.
Cetus Corporation
Ciotti Thomas E.
Fineman Elliott L.
Schain Howard E.
LandOfFree
Method of recovering microbially produced recombinant ricin toxi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of recovering microbially produced recombinant ricin toxi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of recovering microbially produced recombinant ricin toxi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1923647