Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-12-13
1998-06-30
Ketter, James
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4351723, C12Q 168
Patent
active
057732213
ABSTRACT:
The present invention is directed to a method of recovering a biological molecule from a recombinant microorganism produced using a method based on either protoplast fusion or lipofection. The present invention further provides a method of identifying, isolating, or making a biological molecule from a recombinant microorganism and also encompasses the biological molecule produced by a recombinant microorganism. Moreover, the present invention relates to a method of making a recombinant microorganism containing the nucleic acid of a microorganism in an environmental sample, as well as the recombinant microorganisms themselves. Finally, the present invention is directed to a method of making a recombinant library, as well as the recombinant library itself.
REFERENCES:
Matsushima, P. et al., Protoplast Fusion. In A.L. Demain and N.A. Solomon, eds. Manual of Industrial Microbiology and Biotechnology. American Society for Microbiology, Washington, D.C. pp. 170-183. (1986).
Hopwood, D.A., "Genetic Studies With Bacterial Protoplasts," Ann. Rev. Microbiol., vol. 35, pp. 237-272 (1981).
Mirdamadi-Tehrani, J. et al., "Genetic Analysis of Intraspecies Recombinant Formation by Protoplast Fusion with Three Species of Streptomyces," FEMS Microbiology Letters, vol. 36, pp. 299-302 (1986).
Chen, W. et al., "Intergeneric Protoplast Fusion Between Ruminoccoccus albus and an Anaerobic Recombinant, FE7," Applied and Environmental Microbiology, vol. 54, pp. 1249-1253 (1988).
Aono, R. et al., "Genetic Recombination After Cell Fusion of Protoplasts From the Facultative Alkaliphile Bacillus sp. C-125," Microbiology, vol. 40, pp. 3085-3090 (1994).
Rajendran, N., "Expression of the Insecticidal Crystal Protein Gene From a Gram-Positive Bacillus Thuringiensis in a Gram-Negative Pseudomonas Fluorescens Mediated by Protoplast Fusion," FEMS Microbiology Letters, vol. 122, pp. 103-108 (1994).
Prakash, R.K. et al., "Creation of Novel Nitrogen-Fixing Actinomycetes by Protoplast Fusion of Frankia With Streptomyces," Plant Molecular Biology, vol. 10, pp. 281-289 (1988).
Kinashi, H. et al., "Isolation and Characterization of Linear Plasmids From Lankacidin-Producing Streptomyces Species," The Journal of Antibiotics, vol. 47, pp. 1447-1455 (1994).
Yamashita, F. et al. "New Antibiotic-Producing Streptomycetes, Selected by Antibiotic Resistance as a Marker," The Journal of Antibiotics, vol. 38, pp. 58-63 (1985).
Ikeda, Il., "Genetic Engineering of Antibiotic-Producing Microorganisms," In S. Omura, ed., The Search for Bioactive Compounds from Microorganisms. Springer-Verlag, New York, pp. 327-336.
New, R.R.C., ed. Liposomes, A Practical Approach, IRI, Press New York.
Smith, J.G. et al., "Liposomes as Agents of DNA Transfer," Biochimica et Biophysica Acta, vol. 1154, pp. 327-340 (1993).
Nabel, G.J. et al., "Direct Gene Transfer With DNA-Liposome Complexes in Melanoma: Expression, Biologic Activity, and Lack of Toxicity in Humans," Proc. Natl. Acad. Sci., vol. 90, pp. 11307-11311 (1993).
Goyal, K. et al., "Gene Therapy Using DC-Chol Liposomes," J. Liposome Res., vol. 5, pp. 49-60 (1995).
Bakker-Woudenberg, I.A.J.M. et al., "Efficacy of Gentamicin or Ceftazidime Entrapped in Liposomes With Prolonged Blood Circulation and Enhanced Localization in Klebsiella pneumoniae--Infected Ling Tissue," J. Infect. Dis., vol. 171, pp. 938-947 (1995).
Bermudez, L.E. et al., "Treatment of Disseminated Mycobacterium Avium Complex Infection of Beige Mice With Liposome-Encapsulated Aminoglycosides," J. Infec. Dis. 16:1262-1268 (1990).
Boizet, B. et al., "Transfection of Lactobacillus bulgaricus Protoplasts by Bacteriophage DNA," Applied and Environmental Microbiology, vol. 54, pp. 3014-3018 (1988).
Chernyavskii, V.A. et al., "Transfection of Escherichia coli Spheroplasts by Phage DNA Encapsulated in Lipids," Biotekhnologiya, vol. 2, pp. 23-27 (1986).
Lampel, J.S. et al., "Transformation and Transfection of Anthracycline-Producing Streptomycetes," Appl. Environ. Microbiol., vol. 51, pp. 126-131 (1986).
Holubova, I. et al., "Transfer of Liposome-Encapsulated Plasmid DNA to Bacillus subtilis Protoplasts and Calcium-Treated Escherichia coli Cells," Folia Microbiol., vol. 30, pp. 97-100 (1985).
Makins, J.F. et al., "Liposome-Mediated Transformation of Streptomycetes by Chromosomal DNA." Nature 293:671-673 (1981).
McInerney, J.O., "Extraction of Prokaryotic Genomic DNA From Marine Microbial Communities Suitable For Amplification Using The Polymerase Chain Reaction," Int. Revue ges. Hydrobiol., vol. 80, pp. 351-360 (1995).
Frischer, M.E., et al., "Plasmid Transfer to Indigenous Marine Bacterial Populations by Natural Transformation," FEMS Microbiology Ecology, vol. 15, pp. 127-136 (1994).
Lovell, C.R. et al., "Purification of DNA From Estuarine Sediments," Journal of Microbiological Methods, vol. 20, pp. 161-174 (1994).
Stein, J.L. et al., "Characterization of Uncultivated Prokaryotes: Isolation and Analysis of a 40-Kilobase-Pair Genome Fragment From a Planktonic Marine Archaeon," Journal of Bacteriology, vol. 178, pp. 591-599 (1996).
Van Der Vossen, Jos. M.B.M. et al., "Liposome-enhanced transformation of Streptococcus lactis and plasmid transfer by intergenic protoplast fusion of Steptococcus lactis and Bacillus subtilis," FEM SMicrobiology Letters, vol. 49, pp. 323-329 (1988).
Carlson Peter S.
Chmurny Alan B.
Manyak David M.
Quintero Ernesto J.
Ketter James
Oceanix Biosciences Corporation
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